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The assay was performed by heating samples in a Mx3005p qPCR machine (Agilent Technologies, USA) from 25 to 97 C in 1 C min 1 increments for 30 s in an expanding saw-tooth profile, a refinement of a previously described protocol 24

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Mosquitoes apply trophic avoidance by avoiding feeding on hosts in areas where insecticides are extensively used [130, 131]

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Cancer 87 , 11661172 (2002)